2018

A central role for PBP2 in the activation of peptidoglycan polymerization by the bacterial cell elongation machinery. P.D.A. Rohs, J. Buss, S.I. Sim, G.R. Squyres, V. Srisuknimit, M. Smith, H. Cho, M. Sjodt, A.C. Kruse, E.C. Garner, S. Walker, D.E. Kahne, T.G. Bernhardt. PLoS Genet 2018; doi: 10.1371/journal.pgen.1007726. PubMed

A pathway-directed screen for inhibitors of the bacterial cell elongation machinery. J.A. Buss, V. Baidin, M.A. Welsh, J. Flores-Kim, H. Cho, B.M. Wood, T. Uehara, S. Walker, D. Kahne, T.G. Bernhardt. Antimicrob Agents Chemother 2018; doi: 10.1128/AAC.01530-18. PubMed

Outer Membrane Translocon Communicates with Inner Membrane ATPase To Stop Lipopolysaccharide Transport. R. Xie, R.J. Taylor, D. Kahne. J Am Chem Soc 2018; 140:12691-12694. PubMed

A cluster of residues in the lipopolysaccharide exporter that selects substrate variants for transport to the outer membrane. B.R. Bertani, R.J. Taylor, E. Nagy, D. Kahne, N. Ruiz. Mol Microbiol 2018; 109:541-554. PubMed

Novobiocin enhances polymyxin activity by stimulating lipopolysaccharide transport. M.D. Mandler, V. Baidin, J. Lee, K.S. Pahil, T.W. Owens, D. Kahne. J Am Chem Soc 2018; 140:6749-53. PubMed

Cell-based screen for discovering lipopolysaccharide biogenesis inhibitors. G. Zhang, V. Baidin, K. Pahil, E. Moison, D. Tomasek, N.S. Ramadoss, A.K. Chatterjee, C.W. McNamara, T.S. Young, P.G. Schultz, T.C. Meredith, D. Kahne. Proc Natl Acad Sci USA 2018; 115:6834-9. PubMed

Structure of the peptidoglycan polymerase RodA resolved by evolutionary coupling analysis. M. Sjodt, K. Brock, G. Dobihal, P.D.A. Rohs, A.G. Green, T.A. Hopf, A.J. Meeske, V. Srisuknimit, D. Kahne, S. Walker, D.S. Marks, T.G. Bernhardt, D.Z. Rudner, A.C. Kruse. Nature 2018; 556:118-121. PubMed

Membrane potential is required for MurJ function. F.A. Rubino, S. Kumar, N. Ruiz, S. Walker, D. Kahne. J Am Chem Soc 2018; 140:4481-4. PubMed

Antibiotic combinations that enable one-step, targeted mutagenesis of chromosomal genes. W. Lee, T. Do, G. Zhang, D. Kahne, T.C. Meredith, S. Walker. ACS Infect Dis 2018; 4:1007-18. PubMed

Substrate binding to BamD triggers a conformational change in BamA to control membrane insertion. J. Lee, H.A. Sutterlin, J.S. Wzorek, M.D. Mandler, C.L. Hagan, M. Grabowicz, D. Tomasek, M.D. May, E.M. Hart, T.J. Silhavy, D. Kahne. Proc Natl Acad Sci USA 2018; 115:2359-64. PubMed

Lipopolysaccharide is transported to the cell surface by a membrane-to-membrane protein bridge. D.J. Sherman, R. Xie, R.J. Taylor, A.H. George, S. Okuda, P.J. Foster, D.J. Needleman, D. Kahne. Science 2018; 359:798-801. PubMed

Substrate Preferences Establish the Order of Cell Wall Assembly in Staphylococcus aureus. K. Schaefer, T.W. Owens, D. Kahne, S. Walker. J Am Chem Soc 2018; 140:2442-2445. PubMed

2017

Identification of a Functionally Unique Family of Penicillin-Binding Proteins. M.A. Welsh, A. Taguchi, K. Schaefer, D. Van Tyne, F. Lebreton, M.S. Gilmore, D. Kahne, S. Walker. J Am Chem Soc 2017; 139:17727-30. PubMed

 The antibiotic novobiocin binds and activates the ATPase that powers lipopolysaccharide transport. J.M. May, T.W. Owens, M.D. Mandler, B.W. Simpson, M.B. Lazarus, D.J. Sherman, R.M. Davis, S. Okuda, W. Massefski, N. Ruiz, D. Kahne. J Am Chem Soc 2017; 139:17221-4.  PubMed

Outer Membrane Biogenesis.  A. Konovalova, D.E. Kahne, T.J. Silhavy. Annu Rev Microbiol 2017; 71:539-56. PubMed

Peptidoglycan Cross-Linking Preferences of Staphylococcus aureus Penicillin-Binding Proteins Have Implications for Treating MRSA Infections. V. Srisuknimit, Y. Qiao, K. Schaefer, D. Kahne, S. Walker. J Am Chem Soc 2017; 139(29):9791-4. PubMed

Lipid II overproduction allows direct assay of transpeptidase inhibition by beta-lactams. Y. Qiao, V. Srisuknimit, F. Rubino, K. Schaefer, N. Ruiz, S. Walker, D. Kahne. Nat Chem Biol 2017; 13:793-8. PubMed

A fluorescent probe distinguishes between inhibition of early and late steps of lipopolysaccharide biogenesis in whole cells.  E. Moison, R. Xie, G. Zhang, M.D. Lebar, T.C. Meredith, D. Kahne. ACS Chem Biol 2017; 12:928-32. PubMed

Membrane integration of an essential beta-barrel protein prerequires burial of an extracellular loop.  J.S. Wzorek, J. Lee, D. Tomasek, C.L. Hagan, D.E. Kahne.  Proc Natl Acad Sci USA 2017; 114:2598-2603. PubMed

In vitro reconstitution demonstrates the cell wall ligase activity of LCP proteins.  K. Schaefer, L.M. Matano, Y. Qiao, D. Kahne, S. Walker. Nat Chem Biol 2017; 13:396-401. PubMed

 

2016

Identification of residues in the lipopolysaccharide ABC transporter that coordinate ATPase activity with extractor function.  B.W. Simpson, T.W. Owens, M.J. Orabella, R.M. Davis, J.M. May, S.A. Trauger, D. Kahne, N. Ruiz. MBio 2016; 7:e01729-16. PubMed

SEDS proteins are a widespread family of bacterial cell wall polymerases.  A.J. Meeske, E.P. Riley, W.P. Robins, T. Uehara, J.J. Mekalanos, D. Kahne, S. Walker, A.C. Kruse, T.G. Bernhardt, D.Z. Rudner.  Nature 2016; 537:634-8. PubMed

Characterization of a stalled complex on the beta-barrel assembly machine.  J. Lee, M. Xue, J.S. Wzorek, T. Wu, M. Grabowicz, L.S. Gronenberg, H.A. Sutterlin, R.M. Davis, N. Ruiz, T.J. Silhavy, D.E. Kahne. Proc Natl Acad Sci USA 2016; 113:8717-22. PubMed

Lipopolysaccharide transport and assembly at the outer membrane: the PEZ model.  S. Okuda, D.J. Sherman, T.J. Silhavy, N. Ruiz, D. Kahne.  Nat Rev Microbiol 2016; 14:337-45. PubMed

Cofactor bypass variants reveal a conformational control mechanism governing cell wall polymerase activity.  M. Markovski, J.L. Bohrhunter, T.J. Lupoli, T. Uehara, S. Walker, D.E. Kahne, T.G. Bernhardt.  Proc Natl Acad Sci USA 2016; 113:4788-93. PubMed

The mechanism of action of lysobactin.  W. Lee, K. Schaefer, Y. Qiao, V. Srisuknimit, H. Steinmetz, R. Müller, D. Kahne, S. Walker. J Am Chem Soc 2016; 138:100-3. PubMed

 

2015

Lipopolysaccharide transport to the cell surface: periplasmic transport and assembly into the outer membrane.  J.M. May, D.J. Sherman, B.W. Simpson, N. Ruiz, D. Kahne. Phil Trans R Soc B 2015; 370: 20150027. PubMed

Lipopolysaccharide transport to the cell surface: biosynthesis and extraction from the inner membrane. B.W. Simpson, J.M. May, D.J. Sherman, D. Kahne, N. Ruiz. Phil Trans R Soc B 2015; 370: 20150029. PubMed

Inhibition of the beta-barrel assembly machine by a peptide that binds BamD.  C.L. Hagan, J.S. Wzorek, D. Kahne. Proc Natl Acad Sci U S A 2015; 112:2011-6. PubMed

 

2014

A mutant Escherichia coli that attaches peptidoglycan to lipopolysaccharide and displays cell wall on its surface. M. Grabowicz, D. Andres, M.D. Lebar, G. Malojcic, D. Kahne, T.J. Silhavy. eLife 2014; 4:e05334. PubMed

Detection of lipid-linked peptidoglycan precursors by exploiting an unexpected transpeptidase reaction. Y. Qiao, M.D. Lebar, K. Schirner, K. Schaefer, H. Tsukamoto, D. Kahne, S. Walker. J Am Chem Soc 2014; 136:14678-81. PubMed

Reconstitution of peptidoglycan cross-linking leads to improved fluorescent probes of cell wall synthesis.  M.D. Lebar, J.M. May, A.J. Meeske, S.A. Leiman, T.J. Lupoli, H. Tsukamoto, R. Losick, D.Z. Rudner, S. Walker, D. Kahne. J Am Chem Soc 2014; 136:10874-7. PubMed

MurJ is the flippase of lipid-linked precursors for peptidoglycan biogenesis.  L.T. Sham, E.K. Butler, M.D. Lebar, D. Kahne, T.G. Bernhardt, N. Ruiz.  Science 2014; 345:220-2. PubMed

LptE binds to and alters the physical state of LPS to catalyze its assembly at the cell surface.  G. Malojcic, D. Andres, M. Grabowicz, A.H. George, N. Ruiz, T.J. Silhavy, D. Kahne.  Proc Natl Acad Sci U S A 2014; 111:9467-72. PubMed

Decoupling catalytic activity from biological function of the ATPase that powers lipopolysaccharide transport.  D. Sherman, M.B. Lazarus, L. Murphy, C. Liu, S. Walker, N. Ruiz, D. Kahne.  Proc Natl Acad Sci U S A 2014; 111:4982-7. PubMed

Lipoprotein activators stimulate Escherichia coli penicillin-binding proteins by different mechanisms. T.J. Lupoli, M.D. Lebar, M. Markovski, T. Bernhardt, D. Kahne, S. Walker.  J Am Chem Soc 2014; 136:52-5. PubMed

Moenomycin resistance mutations in Staphylococcus aureus reduce peptidoglycan chain length and cause aberrant cell division. Y. Rebets, T. Lupoli, Y. Qiao, K. Schirner, R. Villet, D. Hooper, D. Kahne, S. Walker.  ACS Chem Biol 2014; 9:459-67. PubMed

 

2013

On the essentiality of lipopolysaccharide to Gram-negative bacteria.  G. Zhang, T.C. Meredith, D. Kahne.  Curr Opin Microbiol 2013; 16:779-85. PubMed

D-amino acids indirectly inhibit biofilm formation in Bacillus subtilis by interfering with protein synthesis.  S.A. Leiman, J.M. May, M.D. Lebar, D. Kahne, R. Kolter, R. Losick.  J Bacteriol 2013; 195:5391-5. PubMed

Bam lipoproteins assemble BamA in vitro.  C.L. Hagan, D.B. Westwood, D.E. Kahne.  Biochemistry 2013; 52:6108-13. PubMed

Validation of inhibitors of an ABC transporter required to transport lipopolysaccharide to the cell surface in Escherichia coli.  D.J. Sherman, S. Okuda, W.A. Denny, D. Kahne.  Bioorg Med Chem 2013; 21:4846-51. PubMed

Forming crosslinked peptidoglycan from synthetic Gram-negative Lipid II.  M.D. Lebar, T.J. Lupoli, H. Tsukamoto, J.M. May, S. Walker, D.E. Kahne. J Am Chem Soc 2013; 135:4632-5. PubMed

Tuning the moenomycin pharmacophore to enable discovery of bacterial cell wall synthesis inhibitors.  C.M. Gampe, H. Tsukamoto, E.H. Doud, S. Walker, D. Kahne.  J Am Chem Soc 2013; 135:3776-9. PubMed

The Escherichia coli Lpt transenvelope protein complex for lipopolysaccharide export is assembled via conserved structurally homologous domains. R. Villa, A.M. Martorana, S. Okuda, L.J. Gourlay, M. Nardini, P. Sperandeo, G. Deho, M. Bolognesi, D. Kahne, A. Polissi.  J Bacteriol 2013; 195:1100-8. PubMed

 

2012

Cytoplasmic ATP hydrolysis powers transport of lipopolysaccharide across the periplasm in E. Coli.  S. Okuda, E. Freinkman, D. Kahne.  Science 2012; 338:1214-7. PubMed

Distinct single-cell morphological dynamics under beta-lactam antibiotics.  Z. Yao, D. Kahne, R. Kishony.  Mol Cell 2012; 48:705-12. PubMed

Disulfide rearrangement triggered by translocon assembly controls lipopolysaccharide export.   S.S. Chng, M. Xue, R.A. Garner, H. Kadokura, D. Boyd, J. Beckwith, D. Kahne.  Science 2012; 337:1665-8. PubMed

Regulation of cell size in response to nutrient availability by fatty acid biosynthesis in Escherichia coli. Z. Yao, R.M. Davis, R. Kishony, D. Kahne, N. Ruiz. Proc Natl Acad Sci U S A 2012; 109:E2561-8. PubMed

Regulated assembly of the transenvelope protein complex required for lipopolysaccharide export.  E. Freinkman, S. Okuda, N. Ruiz, D. Kahne.  Biochemistry 2012; 51:4800-6. PubMed

Activation of the Escherichia coli β-barrel assembly machine (Bam) is required for essential components to interact properly with substrate.  D.P. Ricci, C.L. Hagan, D. Kahne, T.J. Silhavy.  Proc Natl Acad Sci U S A 2012; 109:3487-91. PubMed

 

2011

Modular synthesis of diphospholipid oligosaccharide fragments of the bacterial cell wall and their use to study the mechanism of moenomycin and other antibiotics. C. Gampe, H. Tsukamoto, T.S.A. Wang, S. Walker, D. Kahne. Tetrahedron 2011; 67:9771-8. PubMed

Haloduracin a Binds the Peptidoglycan Precursor Lipid II with 2:1 Stoichiometry. T.J. Oman, T.J. Lupoli, T.S. Wang, D. Kahne, S. Walker, W.A. van der Donk. J Am Chem Soc 2011; 133:17544-7. PubMed

The Reconstituted Escherichia coli Bam Complex Catalyzes Multiple Rounds of Beta-Barrel Assembly. C.L. Hagan, D. Kahne.  Biochemistry 2011; 50:7444-6. PubMed

N-Methylimidazolium chloride-catalyzed pyrophosphate formation: Application to the synthesis of Lipid I and NDP-sugar donors. H. Tsukamoto, D. Kahne.  Bioorg. Med. Chem. Lett. 2011; 21:5050-3. PubMed

Transpeptidase-Mediated Incorporation of d-Amino Acids into Bacterial Peptidoglycan. T.J. Lupoli, H. Tsukamoto, E.H. Doud, T.S. Wang, S. Walker, D. Kahne. J Am Chem Soc 2011; 133:10748-51. PubMed

Primer Preactivation of Peptidoglycan Polymerases.  T.S. Wang, T.J. Lupoli, Y. Sumida, H. Tsukamoto, Y. Wu, Y. Rebets, D.E. Kahne, S. Walker. J Am Chem Soc 2011; 133:8528-30. PubMed

Nine enzymes are required for assembly of the pacidamycin group of peptidyl nucleoside antibiotics.  W. Zhang, I. Ntai, M.L. Bolla, S.J. Malcolmson, D. Kahne, N.L. Kelleher, C.T. Walsh. J Am Chem Soc 2011; 133:5240-3. PubMed

Beta-Barrel Membrane Protein Assembly by the Bam Complex.  C.L. Hagan, T.J. Silhavy, D. Kahne.  Annu Rev Biochem 2011; 80:189-210. PubMed

Lipoprotein LptE is required for the assembly of LptD by the β-barrel assembly machine in the outer membrane of Escherichia coli.  G. Chimalakonda, N. Ruiz, S.S. Chng, R.A. Garner, D. Kahne, T.J. Silhavy. Proc Natl Acad Sci USA 2011; 108:2492-7. PubMed

The complex that inserts lipopolysaccharide into the bacterial outer membrane forms a two-protein plug-and-barrel.  E. Freinkman, S.S. Chng, D. Kahne. Proc Natl Acad Sci USA 2011; 108:2486-91. PubMed

 

2010 

Lipoprotein cofactors located in the outer membrane activate bacterial cell wall polymerases.
C. Paradis-Bleau, M. Markovski, T. Uehara, T.J. Lupoli, S. Walker, D.E. Kahne, T.G. Bernhardt. Cell 2010; 143:1110-20. PubMed

Functional and Structural Analysis of a Key Region of the Cell Wall Inhibitor Moenomycin.  S. Fuse, H. Tsukamoto, Y. Yuan, T.S. Wang, Y. Zhang, M. Bolla, S. Walker, P. Sliz, D. Kahne.  ACS Chem Biol 2010; 5:701-11. PubMed

Nonconsecutive disulfide bond formation in an essential integral outer membrane protein.  N. Ruiz, S.S. Chng, A. Hiniker, D. Kahne, T.J. Silhavy.  Proc Natl Acad Sci USA 2010; 107:12245-50. PubMed

Design and synthesis of functionalized trisaccharides as p53-peptide mimics.  K. Sakurai, D. Kahne. Tetrahedron Letters 2010; 51:3724-7. PubMed

The bacterial cell envelope.  T.J. Silhavy, D. Kahne, S. Walker. Cold Spring Harb Perspect Biol 2010; 2:a000414. PubMed

Proteins required for lipopolysaccharide assembly in Escherichia coli form a transenvelope complex.  S.S. Chng, L.S. Gronenberg, D. Kahne.  Biochemistry 2010; 49:4565-7. PubMed

A three enzyme pathway for 2-amino-3-hydroxycyclopent-2-enone formation and incorporation in natural product biosynthesis.  W. Zhang, M.L. Bolla, D. Kahne, C.T. Walsh.  J Am Chem Soc 2010; 132:6402-11. PubMed

Reconstitution of outer membrane protein assembly from purified components.  C.L. Hagan, S. Kim, D. Kahne.  Science 2010; 328:890-2. PubMed

Characterization of the two-protein complex in Escherichia coli responsible for lipopolysaccharide assembly at the outer membrane.  S.S. Chng, N. Ruiz, G. Chimalakonda, T.J. Silhavy, D. Kahne. Proc Natl Acad Sci USA 2010; 107:5363-8. PubMed

Development of an activity assay for discovery of inhibitors of lipopolysaccharide transport.  L.S. Gronenberg, D. Kahne. J Am Chem Soc 2010; 132:2518–9. PubMed

The role of the substrate lipid in processive glycan polymerization by the peptidoglycan glycosyltransferases.  D.L. Perlstein, T.S. Wang, E.H. Doud, D. Kahne, S. Walker.  J Am Chem Soc 2010; 132:48-9. PubMed

 2009

Studying a cell division amidase using defined peptidoglycan substrates.  T.J. Lupoli, T. Taniguchi, T.S. Wang, D.L. Perlstein, S. Walker, D. Kahne. J Am Chem Soc 2009; 131:18230-1. PubMed

Rapid beta-lactam-induced lysis requires successful assembly of the cell division machinery.   H.S. Chung, Z. Yao, N.W. Goehring, R. Kishony, J. Beckwith, D. Kahne. Proc Natl Acad Sci USA 2009; 106:21872-7. PubMed

Complete characterization of the seventeen step moenomycin biosynthetic pathway. B.  Ostash, E.H. Doud, C. Lin, I. Ostash, D.L. Perlstein, S. Fuse, M. Wolpert, D. Kahne, S. Walker. Biochemistry 2009; 48: 8830-41. PubMed

Transport of Lipopolysaccharide Across the Cell Envelope: The Long Road of Discovery. N. Ruiz, D. Kahne, T.J. Silhavy. Nat Rev Microbiol 2009; 9:677-83. PubMed

Using Biological Performance Similarity to Inform Disaccharide Library Design. T. Tanikawa,  M. Fridman, W. Zhu, B. Faulk, I.C. Joseph, D. Kahne, B.K. Wagner, P.A. Clemons. J Am Chem Soc 2009; 131:5075-83. PubMed

2008

A Unifying Nitrososynthase Involved in Nitrosugar Biosynthesis. Y. Hu, A. Al-Mestarihi, C.L. Grimes, D. Kahne, B.O. Bachmann. J Am Chem Soc 2008; 130:15756-7. PubMed

Isolated Peptidoglycan Glycosyltransferases from Different Organisms Produce Different Glycan Chain Lengths. T.S. Wang, S.A. Manning, S. Walker, D. Kahne. J Am Chem Soc 2008; 130:14068-9. PubMed

Structural Analysis of the Contacts Anchoring Moenomycin to Peptidoglycan Glycosyltransferases and Implications for Antibiotic Design. Y. Yuan, S. Fuse, B. Ostash, P. Sliz, D. Kahne, S. Walker. ACS Chem Biol 2008; 3:429-36. PubMed

Identification of Two Inner-membrane Proteins Required for the Transport of Lipopolysaccharide to the Outer Membrane of Escherichia coli.  N. Ruiz, L.S. Gronenberg, D. Kahne, T.J. Silhavy. Proc Natl Acad Sci U S A 2008; 105:5537-42. PubMed

2007

Analysis of Glycan Polymers Produced by Peptidoglycan Glycosyltransferases.  D. Barrett, T.S. Wang, Y. Yuan, Y. Zhang, D. Kahne, S. Walker. J Biol Chem 2007; 282:31964-71. PubMed

The Direction of Glycan Chain Elongation by Peptidoglycan Glycosyltransferases. D.L. Perlstein, Y. Zhang, T.S. Wang, D.E. Kahne, S. Walker. J Am Chem Soc 2007; 129:12674-5. PubMed

Defining the Roles of the Periplasmic Chaperones SurA, Skp, and DegP in Escherichia coli.  J.G. Sklar, T. Wu, D. Kahne, T.J. Silhavy. Genes Dev 2007; 21:2473-84. PubMed

Characterization of Rhodosaminyl Transfer by the AknS/AknT Glycosylation Complex and Its Use in Reconstituting the Biosynthetic Pathway of Aclacinomycin A.  C. Leimkuhler, M. Fridman, T. Lupoli, S. Walker, C.T. Walsh, D. Kahne. J Am Chem Soc 2007; 129:10546-50. PubMed

Kinetic Analysis of Teicoplanin Glycosyltransferases and Acyltransferase Reveal Ordered Tailoring of Aglycone Scaffold to Reconstitute Mature Teicoplanin. A.R. Howard-Jones, R.G. Kruger, W. Lu, J. Tao, C. Leimkuhler, D. Kahne D, C.T. Walsh. J Am Chem Soc 2007; 129:10082-3. PubMed

Structure and Function of an Essential Component of the Outer Membrane Protein Assembly Machine.  S. Kim, J.C. Malinverni, P. Sliz, T.J. Silhavy, S.C. Harrison, D. Kahne. Science 2007; 317:961-4. PubMed

Chemoenzymatic Formation of Novel Aminocoumarin Antibiotics by the Enzymes CouN1 and CouN7.  M. Fridman, C.J. Balibar, T. Lupoli, D. Kahne, C.T. Walsh, S. Garneau-Tsodikova. Biochemistry 2007; 46:8462-71. PubMed

Lipoprotein SmpA is a Component of the YaeT Complex that Assembles Outer Membrane Proteins in Escherichia coli.  J.G. Sklar, T. Wu, L.S. Gronenberg, J.C. Malinverni, D. Kahne, T.J. Silhavy. Proc Natl Acad Sci USA 2007; 104:6400-5. PubMed

Crystal Structure of a Peptidoglycan Glycosyltransferase Suggests a Model for Processive Glycan Chain Synthesis. Y. Yuan, D. Barrett, Y. Zhang, D. Kahne, P. Sliz, S. Walker. Proc Natl Acad Sci USA 2007; 104:5348-53. PubMed

Synthesis of Heptaprenyl-lipid IV to Analyze Peptidoglycan Glycosyltransferases. Y. Zhang, E.J. Fechter, T.S. Wang, D. Barrett, S. Walker, D.E. Kahne. J Am Chem Soc 2007; 129:3080-1. PubMed

2006

The Total Synthesis of Moenomycin A.  J.G. Taylor, X. Li, M. Oberthür, W. Zhu, D. Kahne. J Am Chem Soc 2006; 128:15084-5. PubMed

Degradation and Reconstruction of Moenomycin A and Derivatives:  Dissecting the Function of the Isoprenoid Chain.  M. Adachi, Y. Zhang, C. Leimkuhler, B. Sun, J.V. LaTour, D. Kahne. J Am Chem Soc 2006; 128:14012-3. PubMed

Crystallographic Analysis of an 8-mer p53 Peptide Analogue Complexed with MDM2.  K. Sakurai, C. Schubert, D. Kahne. J Am Chem Soc 2006; 128:11000-1. PubMed

Identification of a Protein Complex that Assembles Lipopolysaccharide in the Outer Membrane of Escherichia coli.  T. Wu, A.C. McCandlish, L.S. Gronenberg, S.S. Chng, T.J. Silhavy, D. Kahne. Proc Natl Acad Sci U S A 2006; 103:11754-9. PubMed

Detection of bacterial toxins with monosaccharide arrays. M.M. Ngundi, C.R. Taitt, S.A. McMurry, D. Kahne, F.S. Ligler. Biosens. Bioelectron. 2006; 21:1195-201. PubMed

Probing the Barrier Function of the Outer Membrane with Chemical Conditionality. N. Ruiz, T. Wu, D. Kahne, T.J. Silhavy. ACS Chem Biol 2006; 1:385-95. PubMed

YfiO Stabilizes the YaeT Complex and is Essential for Outer Membrane Protein Assembly in Escherichia coli. J.C. Malinverni, J. Werner, S. Kim, J.G. Sklar, D. Kahne, R. Misra, T.J. Silhavy. Mol Microbiol 2006; 61:151-64. PubMed

Installation of the Pyrrolyl-2-carboxyl Pharmacophore by CouN1 and CouN7 in the Late Biosynthetic Steps of the Aminocoumarin Antibiotics Clorobiocin and Coumermycin A1. S. Garneau-Tsodikova, A. Stapon, D. Kahne, C.T. Walsh. Biochemistry 2006; 45:8568-78. PubMed

Advances in Understanding Bacterial Outer-membrane Biogenesis. T.J. Silhavy, N. Ruiz, D. Kahne. Nat Rev Microbiol 2006; 4:57-66. PubMed

2005

In Vitro Reconstitution of EryCIII Activity for the Preparation of Unnatural Macrolides. Y. Yuan, H.S. Chung, C. Leimkuhler, C.T. Walsh, D. Kahne, S. Walker. J Am Chem Soc 2005; 127:14128-9. PubMed

A Systematic Investigation of the Synthetic Utility of Glycopeptide Glycosyltransferases. M. Oberthur, C. Leimkuhler, R.G. Kruger, W. Lu, C.T. Walsh, D. Kahne. J Am Chem Soc  2005; 127:10747-52. PubMed

AknT Is an Activating Protein for the Glycosyltransferase AknS in L-Aminodeoxysugar Transfer to the Aglycone of Aclacinomycin A. W. Lu, C. Leimkuhler, G.J. Gatto Jr., R.G. Kruger, M. Oberthur, D. Kahne, C.T. Walsh. Chem Biol. 2005; 12:527-34. PubMed

Characterization of the Aminocoumarin Ligase SimL from the Simocyclinone Pathway and Tandem Incubation with NovM,P,N from the Novobiocin Pathway. M. Pacholec, C.L. Freel Meyers, M. Oberthur, D. Kahne, C.T. Walsh. Biochemistry 2005; 44:4949-56. PubMed

Chemical Conditionality: A Genetic Strategy to Probe Organelle Assesmbly. N. Ruiz, B. Falcone, D. Kahne, T.J. Silhavy. Cell 2005; 121:307-17. PubMed

Identification of a Multi-Component Complex Required for Outer Membrane Biogenesis in Escerichia coli. T. Wu, J. Malinverni, N. Ruiz, S. Kim, T.J. Silhavy, D. Kahne. Cell 2005; 121:235-46. PubMed

Differential Inhibition of S. aureus PBP2 by Glycopeptide Antibiotics.  C. Leimkuhler, L. Chen, D. Barrett, G. Panzone, B. Sun, B. Falcone, S. Donadio, S. Walker, D. Kahne. J Am Chem Soc 2005; 127:3250-1. PubMed

Glycopeptide and Lipoglycopeptide Antibiotics. D. Kahne, C. Leimkuhler, W. Lu, C.T. Walsh. Chem Rev 2005; 105:425-48. PubMed

Tailoring of Glycopeptide Scaffolds by the Acyltransferases from the Teicoplanin and A-40,926 Biosynthetic Operons. R. G. Kruger, W. Lu, M. Oberthuer, J. Tao, D. Kahne, C. T. Walsh. Chem Biol 2005; 12:131-40.  PubMed

Glycosylation of Glycopeptides:  A Comparison of Chemoenzymatic and Chemical Methods. C. Leimkuhler, Z. Chen, R. Kruger, M. Oberthuer, W. Lu, C.T. Walsh, D. Kahne. Tetrahedron: Asymmetry 2005; 16:599-603. ScienceDirect

Comparison of Antigen Constructs and Carrier Molecules for Augmenting the Immunogenicity of the Monosaccharide Epithelial Cancer Antigen Tn. E. Kagan, G. Ragupathi, S. S. Yi, C. A. Reis, J. Gildersleeve, D. Kahne, H. Clausen, S. J. Danishefsky, P. O. Livingston.  Cancer Immunol Immunother 2005; 54:424-30. PubMed

Kinetic Characterization of the Glycosyltransferase Module of Stahylococcus Aureus PBP2. D.S. Barrett, C.E. Leimkuhler, L. Chen, D. Walker, D. Kahne, S. Walker. J Bacteriol 2005; 187:2215-7. PubMed

2004

Use of a Retroinverso p53 Peptide as an Inhibitor of MDM2. K. Sakurai, H.S. Chung, D. Kahne. J Am Chem Soc 2004; 126:16288-9. PubMed

Assembly of Dimeric Variants of Coumermycins by Tandem Action of the Four Biosynthetic Enzymes CouL, CouM, CouP and NovN. C. Freel Meyers, M. Oberthuer, L. Heide, D. Kahne, C. Walsh. Biochemistry 2004; 43:15022-36. PubMed

A Practical Method for the Stereoselective Generation of beta-2-Deoxy Glycosyl Phosphates.  M. Oberthuer, C. Leimkuhler, D. Kahne. Org Lett 2004; 6:2873-6. PubMed

Reconstitution and Characterization of a New Desosaminyl Transferase, EryCIII, from the Erythromycin Biosynthetic Pathway. H. Lee, H. Chung, C. Hang, C. Koshla, C. Walsh, D. Kahne, S. Walker. J Am Chem Soc 2004; 126;9924-5. PubMed

Characterization of NovP and NovN: Completion of Novobiocin Biosynthesis by Sequential Tailoring of the Noviosyl Ring.  C. Freel Meyers, M. Oberthuer, H. Xu, L. Heide, D. Kahne, C. Walsh. Angewandte Chemie Int Ed 2004; 43:67-70. PubMed

Stochastic Detection of Monovalent and Bivalent Protein-Ligand Interactions. S. Howorka, J. Nam, H. Bayley, D. Kahne. Angewandte Chemie Int Ed 2004; 43:842-6. PubMed

Characterization of a Regiospecific Epivancosaminyl Transferase GtfA and Enzymatic Reconstruction of the Antibiotic Chloroeremomycin. W. Lu, M. Oberthuer, C. Leimkuhler, J. Tao, D. Kahne, C. Walsh. Proc Natl Acad Sci USA 2004; 101:4390-5. PubMed

AknK Is an L-2-Deoxyfucosyltransferase in the Biosynthesis of the Anthracycline Aclacinomycin A.  W. Lu, C. Leimkuhler, M. Oberthuer, D. Kahne, C. Walsh. Biochemistry 2004; 43:4548-58. PubMed

2003

Vancomycin Analogues Active Against VanA-Resistant Strains Inhibit Bacterial Transglycosylase Without Binding Substrate. L. Chen, D. Walker, B. Sun, Y. Hu, S. Walker, D. Kahne. Proc Natl Acad Sci USA 2003; 100:5658-63. PubMed

Initial Characterization of Novobiocic Acid Noviosyl Transferase Activity of NovM in Biosynthesis of the Antibiotic Novobiocin. C. Freel Meyers, M. Oberthuer, J. Anderson, D. Kahne, C. Walsh. Biochemistry 2003; 42:4179-89. PubMed

Overcoming Degeneracy in Carbohydrate Recognition. A. Basu, D. Kahne. Angewandte Chemie Int Ed 2003; 42:2504-6. PubMed

2002

Biopolymers: Multifaceted Biopolymers. F. Eckstein, D. Kahne, W. Degrado. Current Opinion in Chemical Biology 2002; 6:805-8. ScienceDirect

Incorporation of Glucose Analogs by Glycosyltransferases GtfE and GtfD from the Vancomycin Biosynthetic Pathway to Generate Variant Glycopeptides. H. Losey, J. Jiang, J. Biggins, M. Oberthuer, X. Ye, S. Dong, D. Kahne, J. Thorson, C. Walsh. Chemistry and Biology 2002; 9:1305-14. PubMed

Structural Requirements for VanA Activity of Vancomycin Analogues. Z. Chen, U. Eggert, S. Dong, S. Shaw, B. Sun, J. LaTour, D. Kahne. Tetrahedron 2002; 58:6585-94. ScienceDirect

The Structural Basis for Induction of VanB Resistance. S. Dong, M. Oberthuer, H. Losey, J. Anderson, U. Eggert, M. Peczuh, C. Walsh, D. Kahne. J Am Chem Soc 2002; 124:9064-5. PubMed

2001

Hybrid Glycopeptide Antibiotics. B. Sun, Z. Chen, U. Eggert, S. Shaw, J. LaTour, D. Kahne. J Am Chem Soc 2001; 123:12722-3. PubMed

Genetic Basis for Activity Differences Between Vancomycin and Glycolipid Derivatives of Vancomycin. U. Eggert, N. Ruiz, B. Falcone, A. Branstrom, R. Goldman, T. Silhavy, D. Kahne. Science 2001; 294:361-64. PubMed

Determining Absolute Configuration in Flexible Molecules: A Case Study. K. Specht, J. Nam, D. Ho, N. Berova, R. Kondru, D. Beratan, P. Wipf, R. Pascal, Jr., D. Kahne. J Am Chem Soc  2001; 123:8961-6. PubMed

Tandem Action of Glycosyltransferases in the Maturation of Vancomycin and Teicoplanin Aglycones: Novel Glycopeptides. H. Losey, M. Peczuh, Z. Chen, U. Eggert, S. Dong, I. Pelczer, D. Kahne, C. Walsh. Biochemistry 2001; 40:4745-55. PubMed

Better Substrates for Bacterial Transglycosylases. X. Ye, M. Lo, L. Brunner, D. Walker, D. Kahne, S. Walker. J Am Chem Soc 2001; 123:3155-6. PubMed

2000 

The Role of Hydrophobic Substituents in the Biological Activity of Glycopeptide Antibiotics. R. Kerns, S. Dong, S. Fukuzawa, J. Carbeck, J. Kohler, L. Silver, D. Kahne. J Am Chem Soc 2000; 122:12608-9. JACS

Hydrogen Abstraction on Photolysis of a Naphthocarborane. A. Bradley, A. Link, K. Biswas, D. Kahne, J. Schwartz, M. Jones, Jr., Z. Zhu, M. Platz. Tetrahedron Letters 2000; 41:8691-4. ScienceDirect

The Molecular Basis for Pyrimidine-Selective DNA Binding: Analysis of Calicheamicin Oligosaccharide Derivatives by Capillary Electrophoresis. K. Biswas, S. Pal, J. Carbeck, D. Kahne, J Am Chem Soc 2000; 122:8413-20. JACS

Calicheamicin-DNA Recognition: An Analysis of Seven Different Drug-DNA Complexes. A. Kalben, S. Pal, A. Andreotti, S. Walker, D. Gange, K. Biswas, D. Kahne. J Am Chem Soc 2000; 122:8403-12. JACS

Design of an Oligosaccharide Scaffold That Binds in the Minor Groove of DNA. H. Xuereb, M. Maletic, I. Pelczer, J. Gildersleeve, D. Kahne. J Am Chem Soc 2000; 122:1883-90. JACS

1990 – 1999

Nonstatistical Binding of a Protein to Clustered Carbohydrates. N. Horan, L. Yan, H. Isobe, G. Whitesides, D. Kahne. Proc Natl Acad Sci USA 1999; 96:11782-6. PubMed

Scavenging Byproducts in the Sulfoxide Glycosylation Reaction: Application to the Synthesis of Ciclamycin. J. Gildersleeve, A. Smith, K. Sakurai, S. Raghavan, D. Kahne. J Am Chem Soc 1999; 121:6176-82. JACS

Vancomycin Derivatives that Inhibit Peptidoglycan Biosynthesis Without Binding D-Ala-D-Ala. M. Ge, Z. Chen, H. Onishi, J. Kohler, L. Silver, R. Kerns, S. Fukuzawa, C. Thompson, D. Kahne. Science 1999; 284:507-11. PubMed

Synthesis of Vancomycin from the Aglycon. C. Thompson, M. Ge, D. Kahne. J Am Chem Soc 1999; 121:1237-44. JACS

DNA-Binding Glycoconjugates. D. Kahne, D. Silva, S. Walker. Bioorganic Chemistry: Carbohydrates Oxford University Press, 1999; 174. ResearchGate

SmI2 Cleavage of Chromomycin A3 Sugars. K. Specht, C. Harris, G. Molander, D. Kahne. Tetrahedron Letters 1999; 40:1855-6. ScienceDirect

Reconstruction of Vancomycin by Chemical Glycosylation of the Pseudoaglycon. M. Ge, C. Thompson, D. Kahne. J Am Chem Soc 1998; 120:11014-5. JACS

Sulfenate Intermediates in the Sulfoxide Glycosylation Reaction. J. Gildersleeve, R. Pascal, Jr., D. Kahne, J Am Chem Soc 1998; 120:5961-9. JACS

Design of Compounds that Increase the Absorption of Polar Molecules. C. Bowe, L. Mokhtarzadeh, P. Venkatesan, S. Babu, H. Axelrod, M. Sofia, R. Kakarla, T. Chan, J. Kim, H. Lee, G. Amidon, S. Choe, S. Walker, D. Kahne. Proc Natl Acad Sci USA 1997; 94:12218-23. PubMed

Polyvalent Binding to Carbohydrates Immobilized on an Insoluble Resin. R. Liang, J. Loebach, N. Horan, M. Ge, C. Thompson, L. Yan, D. Kahne, Proc Natl Acad Sci USA 1997; 94:10554-9. PubMed

Combinatorial Approaches to Carbohydrates. D. Kahne. Current Opinion in Chemical Biology 1997;1:130-5. PubMed

Parallel Synthesis and Screening of a Solid Phase Carbohydrate Library. R. Liang, L. Yan, J. Loebach, M. Ge, Y. Uozumi, K. Sekanina, N. Horan, J. Gildersleeve, C. Thompson, A. Smith, K. Biswas, W. Still, D. Kahne. Science 1996; 274:1520-2. PubMed

Generalizing Glycosylation: Synthesis of the Blood Group Antigens Lea, Leb, and Lex Using a Standard Set of Reaction Conditions. L. Yan, D. Kahne. J Am Chem Soc 1996; 118:9239-48. JACS

Cationic Facial Amphiphiles: A Promising Class of Transfection Agents. S. Walker, M. Sofia, R. Kakarla, N. Kogan, L. Wierichs, C. Longley, K. Bruker, H. Axelrod, S. Midha, S. Babu, D. Kahne. Proc Natl Acad Sci USA 1996; 93:1585-90. PubMed

Sensitivity of Glycopeptide Conformation to Carbohydrate Chain Length. R. Liang, A. Andreotti, D. Kahne. J Am Chem Soc 1995; 116:10395-6. JACS

p-Methoxybenzyl Ethers as Acid-Labile Protecting Groups in Oligosaccharide Synthesis. L. Yan, D. Kahne, Synlett 1995; 523-4. ResearchGate

Strategies for the Design of Minor Groove Binders: A Re-Evaluation Based on the Emergence of Site-Selective Carbohydrate Binders. D. Kahne. Chemistry & Biology 1995; 2:7-12. PubMed

Glycosylation on the Merrifield Resin Using Anomeric Sulfoxides. L. Yan, C. Taylor, R. Goodnow, D. Kahne. J Am Chem Soc 1994; 116:6953-4. JACS

Hydrogen Bonding in Micelle Formation. P. Venkatesan, Y. Cheng, D. Kahne. J Am Chem Soc 1994; 116:6955-6. JACS

Use of Triethylene Glycol to Mimic Oligosaccharides: Design and Synthesis of a Ligand Based on Chromomycin A3. D. Silva, C. Kraml, D. Kahne. Bioorg & Med Chem 1994; 2:1251-9. PubMed

Analysis of Hydroxylamine Glycosidic Linkages:  Structural Consequences of the NO Bond in Calicheamicin. S. Walker, V. Gupta, D. Gange, D. Kahne. J Am Chem Soc 1994; 116:3197-3206. JACS

Chromomycin A3 as a Blueprint for Designed Metal Complexes. D. J. Silva, C. Kraml, D. Kahne. J Am Chem Soc 1994; 116:2641-2. JACS

A Concise Synthesis of the Calicheamicin Oligosaccharide Using the Sulfoxide Glycosylation Method. S. Kim, D. Augeri, D. Yang, D. Kahne. J Am Chem Soc 1994; 116:1766-75. JACS

Isomorphous Binding of Mercury-Substituted Thiosaccharides to Pertussis Toxin Crystals Yields Crystallographic Phases. R. Shigeta, Jr., K. Forest, L. Yan, D. Kahne, C. Schutt. Acta Cryst 1994; D50:71-4. PubMed

NMR Characterization of Calicheamicin g1I Bound to DNA. S. Walker, A. Andreotti, D. Kahne. Tetrahedron 1994; 50:1351-60.

Studies of the 2:1 Chromomycin A3-Mg2+ Complex in Methanol:  Role of the Carbohydrates in Complex Formation. D. Silva, D. Kahne. J Am Chem Soc 1993; 115:7962-70. JACS

Structural Characterization of a Calicheamicin-DNA Complex by NMR. S. Walker, J. Murnick, D. Kahne. J Am Chem Soc 1993; 115:7954-61. JACS

Effects of Glycosylation on Peptide Backbone Conformation. A. Andreotti, D. Kahne. J. Am. Chem. Soc. 1993; 115:3352-3. JACS

A One Step Synthesis of the Ciclamycin Trisaccharide. S. Raghavan, D. Kahne. J Am Chem Soc 1993; 115:1580-1. JACS

The Sugars in Chromomycin A3 Stabilize the Mg+2 Dimer Complex. D. Silva, R. Goodnow, Jr., D. Kahne. Biochemistry 1993; 32:463-71. PubMed

Direct Introduction of CH2OH by Intermolecular Trapping of CO. V. Gupta, D. Kahne. Tetrahedron Letters 1993; 34:591-4. ScienceDirect

Facial Amphiphiles. Y. Cheng, D. Ho, C. Gottlieb, M. Bruck, D. Kahne. J Am Chem Soc 1992; 114:7319-20. JACS

Cleavage Behavior of Calicheamicin g1 and Calicheamicin T. S. Walker, R. Landovitz, W. Ding, G. Ellestad, D. Kahne. Proc Natl Acad Sci USA 1992; 89:4608-12. PubMed

Conformational Analysis of the N-O Bond in the Calicheamicin Oligosaccharide. S. Walker, D. Yang, D. Gange, D. Kahne. J Am Chem Soc 1991; 113:4716-7. JACS

Construction of Glycosidic N-O Linkages in Oligosaccharides. D. Yang, S. Kim, D. Kahne. J Am Chem Soc 1991; 113:4715-6. JACS

Sugars as DNA Binders:  A Comment on the Calicheamicin Oligosaccharide. S. Walker, K. Valentine, D. Kahne. J Am Chem Soc 1990; 112:6428-9. JACS

Preparation of the 4-Ethylamino Sugar of Calicheamicin: Assignment of Absolute Configuration. D. Kahne, D. Yang, M. Lee. Tetrahedron Letters 1990; 31:21-22.  ScienceDirect

1980 – 1989

Glycosylation of Unreactive Substrates. D. Kahne, S. Walker, Y. Cheng, D. Van Engen. J Am Chem Soc 1989; 111:6881-2. JACS

Efficient Regio- and Stereocontrolled Synthesis of Highly Functionalized Polycyclic Systems Using the Diels-Alder Addition of Stannyl-Substituted Vinylketeneacetals. G. Stork, D. Kahne. Israel Journal of Chemistry 1989; 29:243-6. Wiley Online Library

The Use of Alkoxy-Substituted Anomeric Radicals for the Construction of β-Glycosides. D. Kahne, D. Yang, J. Lim, R. Miller, E. Paguaga. J Am Chem Soc 1988; 110:8716-7. JACS

Hydrolysis of a Peptide Bond in Neutral Water. D. Kahne, W. Still. J Am Chem Soc 1988; 110:7529-34. JACS

Substituent Effects on the Stereochemistry of Substituted Cyclohexanone Dimethylhydrazone Alkylations.  An X-ray Crystal Structure of the Lithiated Cyclohexanone Dimethylhydrazone. D. Collum, D. Kahne, S. Gut, R. DePue, F. Mohamadi, R. Wanat, J. Clardy, G. Van Duyne. J Am Chem Soc 1984; 106:4865-9. JACS

Stereocontrol in Homogeneous Catalytic Hydrogenation via Hydroxyl Group Coordination. G. Stork, D. Kahne. J Am Chem Soc 1983; 105:1072-3. JACS

Kinetic Cyanations of Ketone Enolates. D. Kahne, D. Collum. Tetrahedron Letters 1981; 22:5011-4. ScienceDirect

Sodium Permeability and Myocardial Resistance to Cell Swelling During Metabolic Blockade. M. Pine, D. Kahne, B. Jaski, C. Apstein, K. Thorp, W. Abelmann, Am J Physiol (Heart Circ. Physiol. 8) 1980; 239:H31. PubMed